排序方式: 共有139条查询结果,搜索用时 15 毫秒
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Chin-Yo Lin Vinsensius B Vega Jane S Thomsen Tao Zhang Say Li Kong Min Xie Kuo Ping Chiu Leonard Lipovich Daniel H Barnett Fabio Stossi Ailing Yeo Joshy George Vladimir A Kuznetsov Yew Kok Lee Tze Howe Charn Nallasivam Palanisamy Lance D Miller Edwin Cheung Benita S Katzenellenbogen Yijun Ruan Guillaume Bourque Chia-Lin Wei Edison T Liu 《PLoS genetics》2007,3(6)
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P. E. Badenhorst S. Panter R. Palanisamy S. Georges K. F. Smith A. Mouradov J. Mason G. C. Spangenberg 《Molecular breeding : new strategies in plant improvement》2018,38(2):21
Perennial ryegrass is the most important forage grass used in temperate agriculture. Transgenic perennial ryegrass events with altered fructan biosynthesis have the potential not only to increase animal production by improving digestibility of the grasses, but also to increase pasture intake by the animal due to lower neutral detergent fibre (NDF) concentrations. Transgenic perennial ryegrass plants were shown to have increased (P?<?0.05) in fructan concentrations of leaf blades in transgenic T0 events and have thus an increase in water-soluble carbohydrate and in vivo dry matter digestibility concentrations as well as a decrease in the NDF concentration within the plant in spring and summer. These changes in nutritive value have led to an increase in metabolisable energy of up to 1.7 MJ ME·kg DM?1 in selected T0 events compared to FLp418-20 during spring and summer, with no differences in autumn or winter. The field evaluation of these events and the further development of these events using molecular breeding technologies are described in this paper. 相似文献
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Akila Sathyamoorthi Rajesh Palanisamy Mariadhas Valan Arasu Naif Abdullah Al-Dhabi Mukesh Pasupuleti Jesu Arockiaraj 《International journal of peptide research and therapeutics》2018,24(1):143-155
Heat shock cognate 70 (HSC70) is an important evolutionary conserved protein that plays a major role in maintaining the homeostasis and immunity of many organisms. In this study, a HSC70 from Channa striatus was identified from its cDNA library and characterized using bioinformatics and molecular biology tools. CsHSC70 cDNA was 1953 base pair (bp) in length along with an open reading frame which encoded a polypeptide of 650 amino acid residues. Tissue distribution results showed that CsHSC70 was considerably expressed in gill, to a lesser extent in head kidney, blood, spleen and liver and at low level in other tissues. Using C. striatus gill as cell model, effects of fungal, bacterial and poly I:C stimulant on the mRNA levels of CsHSC70 was examined. We also described the antimicrobial features of two peptides namely CsHSC70 A1and CsHSC70 A2 derived from the N-terminal of CsHSC70 protein. CsHSC70 A1 peptide (40 µg/ml) exhibited potent bactericidal activity against Micrococcus luteus cells. Flow cytometric analysis revealed that the M. luteus cells stained with propidium iodide, upon treated with CsHSC70 A1 at the concentration of 40 µM/ml showed 38% survival compared to its control (99.6%). It seems that CsHSC70 A1 peptide shows antimicrobial activity against M. luteus through membrane disruption. Additionally, scanning electron microscope (SEM) observation confirmed that CsHSC70 A1 peptide treatment completely damaged and destructed the M. luteus cells. Taken together, these findings suggest that CsHSC70 A1 peptide could be a safe and potential therapeutic molecule substitute to antibiotics in various clinical fields. 相似文献
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K. Palanisamy 《Photosynthetica》1999,36(4):635-638
Response of net photosynthetic rate (P
N), stomatal conductance (g
s), intercellular CO2 concentration (c
i), and photosynthetic efficiency (Fv/Fm) of photosystem 2 (PS2) was assessed in Eucalyptus cladocalyx grown for long duration at 800 (C800) or 380 (C380) μmol mol-1 CO2 concentration under sufficient water supply or under water stress. The well-watered plants at C800 showed a 2.2 fold enhancement of P
N without any change in g
s. Under both C800 and C380, water stress decreased P
N and g
s significantly without any substantial reduction of c
i, suggesting that both stomatal and non-stomatal factors regulated P
N. However, the photosynthetic efficiency of PS2 was not altered.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
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We are reporting the selective, sensitive and stable determination of L-cysteine (CY) at physiological pH (pH=7.2) using a gold-aminomercaptothiadiazole core-shell nanoparticles (p-GAMCS NPs) film modified GC electrode. The p-GAMCS NPs film was fabricated on GC electrode by potentiodynamic method using 5-amino-2-mercapto-1,3,4-thiadiazole stabilized gold nanoparticles (AMT-AuNPs). The fabricated p-GAMCS NPs film was characterized by cyclic voltammetry and atomic force microscopy (AFM) techniques. The AFM image of the p-GAMCS NPs film showed that it contains a homogeneously distributed AuNPs with a spherical shape of ~10 nm. The p-GAMCS NPs film modified GC electrode was exploited for the determination of CY. The bare GC electrode failed to show any response for CY (pH=7.2) whereas p-GAMCS NPs film on GC electrode showed a well-defined oxidation peak for CY at 0.51 V. Further, p-GAMCS NPs film modified electrode successfully resolved the voltammetric signals of ascorbic acid (AA) and CY with a peak separation of 500 mV. This is the first report for the large voltammetric peak separation between CY and AA to the best of our knowledge. The amperometric current was increased linearly from 10 nM to 140 nM CY with a detection limit of 3 pM (S/N=3). The present modified electrode showed better recoveries for spiked CY into the human blood serum and urine samples. 相似文献
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Daniel John Womac Arun Prathap Palanisamy Rene Eslick Dennis Kenneth Schimpf Kenneth David Chavin 《PloS one》2013,8(1)
Autologous tissue transfer, in addition to replacing tissue that was lost during injury or surgery, offers women an excellent option to improve cosmetic appearance and self-confidence following mastectomy due to breast cancer. However, flap necrosis is a complication in obese patients undergoing this procedure. We created a mouse model to study the flap-related complications that leads to decreased flap survival in autologous breast reconstruction.